The use of oral fluid from pig populations for the diagnosis and monitoring of infectious disease
Research partner: Newcastle University
Sponsor: AHDB Pork-funded studentship (Lorna Dawson)
Duration: 2011 - 2014
Aims and objectives
- To develop diagnostic testing for the detection of European strains of PRRSv and Salmonella infection in UK pig herds, using oral fluid (OF)
- To find out how to get good quality oral fluid samples that truly represent the total population for large group sizes of pigs.
Findings to date
- Provision of multiple cotton ropes may improve representation of pen-based OF samples in weaned, but not finishing, pigs in straw-based systems
- A single cotton rope can result in a pen sample representative of 40% of the total population, approximately four times more than current blood testing recommendations
- FTA cards* may provide a simple and safe means of storing/shipping porcine OF for PRRSv testing without the need for chilling
- Porcine OF shows good, but not total, agreement with blood serum for anti-PRRS antibody testing
- The next stage of the project is serial OF collection following weaned pens through growth for anti-PRRSv antibody detection and real time PCR testing of dry stored field OF to validate FTA card* method for PRRSv monitoring.
* Flinders Technology Associates (FTA) cards: bind and store nucleic acids at ambient temperatures.