Identification and characterisation of Salmonella enterica serovar Typhimurium factors playing a role in the colonisation of the porcine gut
Research partner: University of Edinburgh
Sponsor: AHDB Pork-funded studentship (Johanna Elvidge)
Duration: 2007 –2010
Aims and objectives
- To identify the roles of different Salmonella Typhimurium binding factors in colonisation of the pig intestine
- To modify the S. Typhimurium vaccine strain (VacT) so that vaccinated pigs can be differentiated from infected animals.
- The role of flagella in interaction of S. Typhimurium to porcine intestinal epithelium was tested and the flagella mutant strains exhibited reduced binding to porcine intestinal epithelial cells
- Purified flagella proteins were also shown to bind porcine intestinal epithelial cells
- The vaccination of pigs with Salmonella flagella provided some protection against challenge
- This project has identified flagella as one potential subunit of a multivalent subunit vaccine to help control salmonellosis in the porcine reservoir.