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Photo of Identification and characterisation of Salmonella enterica serovar Typhimurium factors playing a role in the colonisation of the porcine gut

Steve Dunkley

Head of Knowledge Exchange (Interim)

AHDB Pork KE

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Identification and characterisation of Salmonella enterica serovar Typhimurium factors playing a role in the colonisation of the porcine gut

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Research partner: University of Edinburgh

Sponsor: AHDB Pork-funded studentship (Johanna Elvidge)

Duration: 2007 –2010 

Aims and objectives

  • To identify the roles of different Salmonella Typhimurium binding factors in colonisation of the pig intestine
  • To modify the S. Typhimurium vaccine strain (VacT) so that vaccinated pigs can be differentiated from infected animals. 

Findings

  • The role of flagella in interaction of S. Typhimurium to porcine intestinal epithelium was tested and the flagella mutant strains exhibited reduced binding to porcine intestinal epithelial cells
  • Purified flagella proteins were also shown to bind porcine intestinal epithelial cells
  • The vaccination of pigs with Salmonella flagella provided some protection against challenge
  • This project has identified flagella as one potential subunit of a multivalent subunit vaccine to help control salmonellosis in the porcine reservoir. 

Further information

Click here to download full thesis