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Georgina Crayford

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Georgina CrayfordThe infection biology of pig-associated Salmonella

Georgina Crayford - Liverpool University
Duration - 2010-2014 

Georgina has an interest zoonotic diseases and is particularly interested in pig diseases. She is keen to continue working in the pig industry once she has completed her PhD.

Project Aims and Objectives
To determine the phenotype of monophasic and biphasic strains of Salmonella Typhimurium phage type DT193; to characterise the interaction between DT193 isolates and a porcine intestinal epithelial cell line (IPEC-1) including adhesion and invasion; to determine the physiological response of host IPEC-1 cells to DT193 infection

The overall objective is to add to the current limited knowledge of the pathogenicity of monophasic S. Typhimurium DT193 isolates and determine the reasons behind their rapid and worldwide spread in recent years.

Potential Benefit to Industry:
Monophasic isolates of S. Typhimurium are being isolated with increasing frequency from both humans and pigs. They are now the 2nd most common serotype isolated from pigs and the 3rd most common isolated from humans in Europe.

There is increasing evidence from the veterinary profession that infection with monophasic Salmonella is causing clinical disease in pigs, unlike normal Salmonella infection in pigs which is usually asymptomatic. This is cause for concern to pig farmers whose herd health and productivity could be affected. There is also concern for public health given the zoonotic potential of these strains and their resistance to multiple antimicrobials.

Salmonella attaching to and invading porcine intestinal epithelial cells. DAPI blue fluorescent staining of DNA: large round structures are cell nuclei, small structures are bacterial cellsDownloadable Documents